Plans to erect a two-storey detached garden annex on the site of the Coach House at Giffen near Dalry had been refused in November by North Ayrshire Council.
An appeal was submitted to the Local Review Body, which considered it last week.
The building would have stood 20 metres distant from the main property, which is now a family home.
But planning officials said that the design of the proposed building would be unsympathetic to the setting, character, and appearance of the category C listed building.
The site currently consists of a large former stable block building set within a wooded area that forms part of the estate associated with Giffen House, located to the north-east.
The site is accessible from the C109 road to the west via a private track. The site is well enclosed by woodland and is set back from the public road.
Planning permission and listed building consent were granted last year for alterations and an extension to Coach House, including the erection of a detached garage/car port building. The consents were applied for retrospectively as the work had already taken place.
Committee chair Robert Foster proposed refusing the appeal, and this was unanimously agreed by councillors.
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